- Adler Planetarium and Astronomy Museum
 - Interprets the exploration of the Universe for the broadest possible audience and hosts one of the finest collections of early astronomical instruments in the world. - Chicago Academy of Sciences - Nature Museum
- A fun place where children, families, school groups, and adults can explore nature and science.
- Chicago Center for Book and Paper Arts
- Devoted to the arts and crafts related to handmade paper and books. Offers credit and community classes in papermaking, printing, paper sculpture, bookbinding, and more.
- Chicago Children's Museum
- Chicago Historical Society
- Features information on Chicago and early Illinois history displayed in many exhibits and available to researchers.
- Guide to Museums in Chicago
- Hancock Observatory, The
- See the city like you never have before. Oh, and check out stars and planets too.
- International Museum of Surgical Science
- Portrays many of the personalities, failures and breakthroughs that have shaped modern surgery. Allows you to step back in time to the 1800s to become either a survivor or a fatality as a result of early American medical care.
- Leather Archives & Museum
- Dedicated to preserving a record of the leather/BDSM/fetish community and its lifestyles, achievements, and history.
- Museum of Science and Industry (1)
- National Vietnam Veteran Art Museum
- Dedicated to collecting, cataloging, preserving, and displaying works of art created by Americans who served in the armed forces of the Vietnam War.
- Peace Museum, The
- Presents exhibits designed motivate individuals to achieve creative solutions to the problem of violence.
- Polish Museum of America
- Exhibits the art, history, and culture of Poland and the Polish-American Community.
- Viet Nam War Museum
- To honour all who served, veterans, their families, and others in any capacity through exhibits, original writings, historical documents and pictures.
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